Description
Omicron Variant Detection Kit
PRODUCT BRAND: BIOPERFECTUS TECHNOLOGIES
Omicron Variant Detection Kit
CATEGORIES: BIOPERFECTUS TECHNOLOGIES , ACADEMIC AND RESEARCH , COVID PCR ANALYSIS , BIOTECHNOLOGY , COVID-19 , FEATURED OF THE MONTH , LABORATORIES AND HOSPITALS TAGS: BIOPERFECTUS TECHNOLOGIES , KIT VARIANTS
- ANDiS Viral RNA
- Auto Extraction &
- Purification Kit
- Applied Biosystems
- 7500 Real-Time PCR
- Instrument system
- (Thermo Fisher
- Scientific)
- Automated Nucleic
- Acid Extraction
- System ANDiS 350
- Qiagen DSP Viral
- RNA Mini Kit
DESCRIPTION
This kit is used for the in vitro qualitative detection of ORF1ab and S-gene of SARS-CoV-2 in throat swabs, nasopharyngeal swabs and sputum. This kit can also be used to detect mutations E484A, N679K, L981F, 69-70del and H655Y in the S gene of SARS-CoV-2.
The B. 1.1.529 variation is quite different from other variants, with more than 30 mutations in the spike protein (protein S) and about 50 mutations in the entire genome. It is likely to be more infectious, toxic, and immune to escape.
This kit adopts the amplification refractory mutation system (ARMS) technique to detect the mutations. The primers for the ORF1ab gene and the E484A, N679K, L981F, 69-70del and H655Y mutations of the S gene are specifically designed according to the SARS-CoV-2 genome. Probes for ORF1ab, E484A, N679K, L981F, 69-70del and H655Y are labeled with different fluorescent dyes respectively and mutations will be detected by examining the ∆Ct between the target mutation gene and ORF1ab.
This kit contains an internal control (RNase P) to monitor sample collection, transport and extraction to avoid a false negative result.
FEATURES
Technology: PCR-multiplex in real time
Target region: ORF1ab gene, mutations E484A, N679K, L981F,
69-70del, H655Y gene S
Internal Control (IC): RNase P
Rehearsal time: 72 minutes
Clinical specificity: 7%
Lower detectable limit: 500 copies/mL
Reaction volume: 50 uL (30 uL PCR mix-20 uL sample)
Storage temperature: -20±5ºC
Transport temperature: -25~8ºC
Presentation : 50 tests/kit.
COMPONENTS PER KIT
COMPONENTS
VOLUME CONTENT
Buffer for RT-PCR
750uL/vial x2 Tris Hydroxymethyl Aminomethane, Potassium Chloride, Magnesium Chloride, Mixed Nucleotides
Enzyme mix for RT-PCR 1000uL/vial x2
Reverse transcriptase, Rnasin, Taq DNA polymerase
reaction mixture A 250μL/vial ×1
Primers and probes E484A, N679K and ORF1ab
reaction mix B
250μL/vial ×1 L981F, 69-70del, H655Y and RNase P primers and probes
positive control 500uL
Virus-like particles of ORF1ab, S genes and internal control fragments.
Negative Control 500uL
virus-like particles
- delta-variant-mutation-pcr-test-kit
- Covid-19 Mutation/Variant Detection Kit
- bioperfectus-pcr-test-kit
- Delta Variant Detection Kit for Covid-19
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| - The COVID-19 diagnostic tests approved by the Ministry of Health, Labour and Welfare after March 2020 with prior scientific review by the PMDA |
| - The COVID-19 diagnostic tests approved by the Therapeutic Goods Administration (TGA) for inclusion on the Australian Register of Therapeutic Goods (ARTG) on the basis of the Expedited TGA assessment |
| - The COVID-19 diagnostic tests listed on the French government website and under the control of the French Health Authority ANSM |
| - The decisions taken based on the Canada’s Minister of Health interim order (IO) to expedite the review of these medical devices, including test kits used to diagnose COVID-19; |
| - The United States Food and Drug Administration’s (USFDA) general recommendations and procedures applicable to the authorization of the emergency use of certain medical products under sections 564, 564A, and 564B of the Federal |
| - WHO Prequalification decisions made as per the Emergency Use Listing (EUL) procedure opened to candidate in vitro diagnostics (IVDs) to detect SARS-CoV-2; |
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