Ammonia Assay Archives - Gen9 Genetics

Summary: Amidases (L-asparaginase and L-glutaminase) catalyze the process of deamination of L-asparagine and L-glutamine to their corresponding acid form with release of ammonia. Both enzymes are considered one of the most important biomedical and biotechnologically groups of enzymes, in addition to their international contribution as important commercial products. L-asparaginase and L-glutaminase have received more attention as antileukemic agents for the treatment of acute lymphoblastic leukemia (ALL) and other types of cancer.

On the other hand, these enzymes are also used in food manufacturing for their hydrolysis effect and it is a possible way to decrease the amount of free L-asparagine in the preliminary ingredients of food processing, thus minimizing the imminent risk of causing neurotoxic and carcinogenic acrylamide compounds. which was formed when food was heated above 120 ° C. Glutamic acid and aspartic acid are important amino acids in food processing that achieve delicious, fine, sour and umami taste in addition to their nutritional importance for food. A recent review looks at the mode of action of L-asparaginase and L-glutaminase. In addition, this review lists the sources of L-asparaginase and L-glutaminase, the optimization of enzyme production, and the uses of the two enzymes in cancer therapy and other industrial purposes.