Urea Assay Archives - Gen9 Genetics
Sensitive and precise. Use 5 μl samples. Linear detection range of 0.08 mg / dL (13 μM) to 100 mg / dL (17 mM) of urea in a 96-well plate assay.
• Simple and high performance. The procedure involves the addition of a single working reagent and incubation for 20 min. It can be easily automated as a high throughput assay for thousands of samples per day.
• Greater stability and versatility of reagents. The optimized formulation has greatly improved signal and reagent stability. 96-well plate or cuvette assay.
• Low interference in biological samples. No pretreatments are needed. The assays can be performed directly on raw biological samples, that is, in the presence of lipids and proteins.
Urea is produced mainly in the liver and is secreted by the kidneys. Urea is the main end product of protein catabolism in animals. It is the primary vehicle for removing toxic ammonia from the body. The determination of urea is very useful for the clinician to evaluate the renal function of patients. In general, elevated urea levels are associated with nephritis, renal ischemia, urinary tract obstruction, and certain extrarenal diseases, eg, congestive heart failure, liver disease, and diabetes.
Decreased levels indicate acute liver failure or may be the result of overly vigorous parenteral fluid therapy. Simple, direct, and automation-ready procedures for measuring BUN blood urea nitrogen or urea concentration in biological samples are becoming popular in drug discovery and research. The BioAssay Systems Urea Test Kit is designed to measure urea directly in biological samples without any pretreatment. The improved Jung method uses a chromogenic reagent that forms a specifically colored complex with urea. The intensity of the color, measured at 520 nm, is directly proportional to the concentration of urea in the sample. The optimized formulation substantially reduces substance interference in raw samples.