The pET-24a-d(+) vectors carry an N-terminal T7•Tag® sequence plus an optional C-terminal His•Tag® sequence. These vectors differ from pET-21a-d(+) only by their selectable marker (kanamycin vs. ampicillin resistance).
Note that the sequence is numbered according to the pBR322 convention, so the T7 expression region is inverted on the vector map (TB070). The f1 origin is oriented such that infection with helper phage will produce virions containing single-stranded DNA corresponding to the coding strand.
The pET vectors are supplied as purified plasmid DNA (10 µg). Each order of pET DNA also includes an induction control strain (supplied as a glycerol stock). If you need additional information, please contact technical support.
This product is sold for internal research use only. Any commercial use of this product, its components and/or derivatives thereof (including but not limited to proteins produced using the product or its components) (collectively and hereinafter the "EMD Product") requires the signing of a written commercial agreement use with EMD Millipore Corporation or its successor in importance.
Commercial use includes, but is not limited to:
(1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EHR Product to provide services, information or data to third parties for a fee; (3) use of the EHR Product for therapeutic, diagnostic, or prophylactic purposes (including as part of a device, chip, test, or other product); or (4) resale of the EHR product, whether or not such EHR product is resold for research purposes. Nothing herein is deemed to represent or guarantee that additional third party rights are not required to use the EMD product.
For questions about these usage restrictions
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