dna template strand sequencing
Difference between template and encoding thread
Leave a Comment / Science / By Supriya N
The difference between the template and the encoding string is mainly due to the following properties: directional polarity and function. Both the template and the coding strand are the two distinct strands of double-stranded DNA, with the former serving as the basis for transcribing the mRNA and the latter determining the correct base sequence of the mRNA.
Directional Polarity: The template strand moves from the 3 'end to the 5' end, while the coding strand moves in the opposite direction to the template strand, i.e. from the 5 'end to the 3 'end.
Base sequence: The base sequence of the template strand is complementary to both the coding strand and the mRNA strand. In contrast, the base sequence of the coding strand is similar to the new mRNA strand except for one change (uracil replaces thymine). In this session, we will mainly focus on the key differences between the template and the encoding string along with the comparison chart. In addition to this, you will know the definition and examples of the two.
Template thread definition
The template strand is one of the DNA strands whose base sequence helps to build mRNA through complementary base sequencing. The template strand or "antisense strand" runs in the 3 to 5 foot direction, opposite the coding strand. It contains nucleotide sequences complementary to the transcribed mRNA.
After transcription, the mRNA undergoes certain post-transcriptional modifications before becoming mature mRNA. The template strand also contains "Anticodons" that carry triplet codes or triplet nucleotide sequences complementary to the anticodon sequence of the t-RNA.
Anti-coding aids in the binding of the specific amino acid to t-RNA to form a protein or peptide chain through the assistance of rRNA. An RNA polymerase reads the template strand to make an RNA transcript by recognizing the promoter sequences or genes. Therefore, RNA polymerase is the one that decides the initiation of transcription and the termination of the translation process.
Example
Suppose that the template strand carries the sequence of the 5'- A T C G C G T A-3 'gene. RNAP will bind to the promoter region of the DNA sequence first and promote transcription. By binding RNAP to the promoter site, the template strand will be transcribed to form the primary mRNA transcript.
Definition of encoding strand
It is one of the DNA strands with a quadratic base sequence with the primary mRNA or the transcribed mRNA. The mRNA base sequence similar to the coding strand will have the same nucleotide bases, except thymine. In mRNA, uracil is the nitrogen base that replaces thymine.
The encoding or "sense strand" runs in a 5'-3 'direction, opposite the template strand. Since RNAP uses the template strand to transcribe the mRNA, the other strand will be the sense strand that will form a complementary strand to the template strand. It contains triplet codons, which code for the specific amino acid to build proteins through translation of mRNA.
Example
Suppose that the template strand carries the sequence of the 5'- A T C G C G T A-3 'gene. The coding string will produce complementary pairs relative to the template string according to the Watson and Crick model. Therefore, the base sequence of the sense strand will be 3'- T A G C G C A T-5 '. RNAP will bind to the promoter region of the DNA sequence and promote the transcription process. By binding RNAP to the promoter site, the template strand will be transcribed to form the primary transcript with a 3'- U A G C G C A U-5 'base sequence.
Conclusion
Both sense and antisense strands of DNA coordinate to transcribe RNA and translate proteins. The two strands of DNA are sometimes called the Watson and Crick strand, after the two scientists Watson and Crick who gave the model for double-stranded DNA.