panel tactil Archives - Gen9 Genetics

Chemical and optogenetic methods for the post-translational control of protein function have allowed the modulation and engineering of cellular functions. However, most of these methods only confer single-input and single-output control. To increase the diversity of post-translational behaviors that can be programmed, we created a system based on a single protein receptor that can integrate multiple drug inputs, including approved therapies. Our system translates drug inputs into various outputs using a set of reader proteins designed to provide variable receptor protein dimerization states. We show that our single receptor protein architecture can be used to program a variety of cellular responses, including gradual and proportional dual output control of mammalian cell signaling and transcription. We apply our tools to assess the competitive activities of Rac and Rho GTPases to control cell morphology. Our versatile toolkit will allow researchers to program mammalian cellular processes post-translationally and design cell therapies.