HemogloBind™ Hemoglobin Depletion From Hemolyzed Serum/Plasma

Hemoglobin Removal and Capture

• Has a high degree of specificity for hemoglobin, without cross-reacting with other proteins or analytes

• Suitable for

  • Hemolyzed serum/plasma

  • Whole Blood, Dried Blood Cards (DBS) or Erythrocyte lysates

  • Tissue Homogenates, compatible with RIPA buffer

• Applications in analytical interferences, enzyme monitoring, proteomics

• Species Agnostic

• Over 30 citations including analysis in cellular thermal shift assay (CETSA), LC-MS proteomics, Hemoglobin derivatives, Western blot, enzyme activity, ELISA

• Related product NuGel™HemogloBind™ comes in a dry powder format, compatible with high throughput 96-well automation, see https://www.biotechsupportgroup.com/NuGel-HemogloBind-Hemoglobin-Capture-Reagent-p/np-ho.htm

Poly-electrolytes are polymers with repeating units of stationary charges. HemogloBind™ is derived from insoluble elastomeric poly-electrolytes that bind proteins through an empirically derived chemistry combining elements of polymer composition, and cross-linking architecture. As with bio-polymers like DNA and Heparin, governing their reactivity is the spatial presentation of electrostatic groups along a flexible polymer chain.

HemogloBind™ does not cross react with most common serum components, making it an excellent tool in numerous applications. These include analytical protocols where optical interference is problematic. Hemoglobin variants, as in thalassemia, bind with differential affinity towards HemogloBind™, though this has not been fully evaluated. For purification and/or analysis of hemoglobin, a modest elevation in pH will facilitate desorption from the polymer.