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GMD5501-GMP 50kU (200 μL, 250U/μL)

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50kU
$390
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Description

Overview

Taq DNA Polymerase is the robust standard enzyme for the amplification of DNA fragments up to 3 kb in PCR.
  • Obtain consistent results.
    Rely on the robust reaction performance and the lot-to-lot consistency of this product.
  • Stay ahead of regulatory requirements.
    High quality manufacturing, quality control and documentation according to GMP (Good Manufacturing Practice) regulations.

Application

Use Taq DNA Polymerase, GMP Grade, 5 U/μl, for:
  • Routine PCR and RT-PCR applications
  • Amplification of DNA fragments up to 3 kb from various sources of DNA
  • Labeling of DNA with modified nucleotides (e.g., DIG-dUTP, biotin-dUTP, fluorescein-dUTP)
  • Combination with dUTP and Uracil-DNA Glycosylase for prevention of carryover contamination between PCR reactions
  • Manufacture of amplification mixtures for applications with regulatory requirements (e.g.,in vitro diagnostics, quality control)

Properties

Taq DNA Polymerase is the recombinant full-length version of the thermostable enzyme from the eubacterium Thermus aquaticus BM, expressed in E. coli.
Enzyme acivities: Highly processive 5'-3' DNA polymerase; double-strand specific 5'-3' exonuclease; no 3'-5' exonuclease activity
pH optimum: Approximately 9.0 (+20°C)
Temperature optimum: Approximately +75°C
Half life at +95°C: Approximately 40 minutes
Substrates: Incorporates dNTP, dUPT, dITP, various labeled or modified nucleotides (200 μmol/L each is recommended of normal dNTP, increased concentrations of variants)
Divalent ion requirement: Mg2+ (1.5 mmol/L standard concentration)
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