GH4500, GH4507, GH4502, Proteinase K (GMP Grade, Normal Liquid & For NGS)
- SKU:
- UPC:
- MPN:
Description
Overview
Recombinant Proteinase K in PCR Grade is a universal tool for the preparation of nucleic acid templates. Proteinase K, originally isolated from the fungus Tritirachium album, is a recombinant enzyme expressed in Pichia pastoris. It is a highly active serine endopeptidase related to subtilisin that does not exhibit any pronounced cleavage specificity.
Therefore, recombinant, PCR grade proteinase K is a universal tool for template preparation. The amino acid sequence and molecular structure of the recombinant enzyme and the native protease are identical. However, the recombinant proteinase K production process guarantees an enzyme of extraordinary reliability and purity that meets all the requirements of diagnostic manufacturers.
Particular emphasis has been placed on the low DNA content of the enzyme preparation, which makes the recombinant, PCR-grade Proteinase K ideal for isolating PCR and RT-PCR templates.
- Maximize the yield of target nucleic acids.
- Proteinase K is rigorously tested for the absence of nucleases.
- Experience stability and security.
- The solution is very stable and can be stored at room temperature for at least 18 months. The handling of the solution is safe and flexible.
Application
Recombinant, PCR grade proteinase K digests native proteins very efficiently. This enzyme can be used to rapidly inactivate endogenous RNases and DNases during nucleic acid isolation. Proteinase K is particularly suitable for the isolation of native RNA and DNA from tissues and cell lines.
The enzyme promotes cell lysis by activating a bacterial autolytic factor. Proteinase K is also used to:
- Analysis of membrane structures by modifying proteins and glycoproteins on the cell surface.
- Cell debris removal during colony survey preparation.
- Treatment of tissue sections to ensure efficient probe infiltration during in situ hybridization.
Related Products
![Guanosine-5’-monophosphate [GMP],disodium salt Guanosine-5’-monophosphate [GMP],disodium salt](https://cdn11.bigcommerce.com/s-i3vkl74ckd/images/stencil/590x590/products/623/912/bio-basic__43268.1641692136__81459.1641692351__97735.1641965439.png?c=1)
Guanosine-5’-monophosphate [GMP],disodium salt
Bio Basic

PEP-K
Bio Basic

Proteinase K
Bio Basic

Proteinase K, 20mg/mL
Bio Basic
Customers Also Viewed

GMD5501-GMP 50kU (200 μL, 250U/μL)

UnScience Antigen Test 25 tests
GENTAUR

GMD 2101 dNTP mix (25mM each) 10ml

GH7001 BstDNA Polymerase 1.0

GMD3501, 3502 , 3503 Nucleic Acid Extraction Kits

EZ-10 Empty Column without filter, with collection tube & O-ring
Bio Basic

EZ-10 Spin Column Viral Total RNA Extraction Kit
Bio Basic
UltraNuclease

Unscience Antigen Self Test Kit 1 test
GENTAUR

SDS Blue Staining
GENTAUR

EZ-10 Spin Column Viral DNA Mini- Preparations Kit
Bio Basic

EZ-10 Spin Column Yeast Genomic DNA Mini- Preparations Kit
Bio Basic

rProtein A Beads 4FF
Bio Basic

One Tube Viral DNA Isolation Buffer
Bio Basic

YM Agar
Bio Basic

SARS-CoV-2 RT-PCR Detection Kit (COVID-19)
Bio Basic

IL-36β(157a.a.), human
Bio Basic

PEP-K
Bio Basic

2mL Deep Well -Sterile for VT9100
Bio Basic

Plasmid Miniprep Kit (100 Preparations)
Bio Basic

Proteinase K
Bio Basic

MagicMag Viral RNA Kit
Bio Basic

One-Tube Swab DNA Extraction Kit
Bio Basic

EZ-10 Spin Column Soil DNA Mini- Preparations Kit
Bio Basic

VEGF165, rat
Bio Basic

PBS Blocking buffer with BSA
Bio Basic

Proteinase K, 20mg/mL
Bio Basic

96 well DNA plate with membrane (960ul each well)
Bio Basic

Rapid Fungal RNA Extraction Kit
Bio Basic
![Guanosine-5’-monophosphate [GMP],disodium salt Guanosine-5’-monophosphate [GMP],disodium salt](https://cdn11.bigcommerce.com/s-i3vkl74ckd/images/stencil/590x590/products/623/912/bio-basic__43268.1641692136__81459.1641692351__97735.1641965439.png?c=1)
Guanosine-5’-monophosphate [GMP],disodium salt
Bio Basic

Rapid Viral RNA Extraction Kit
Bio Basic

One-Tube Tissue DNA Extraction Kit
Bio Basic

One-tube Plan Genomic DNA Extraction Kit
Bio Basic

Agar C
Bio Basic

CNTF, rat
Bio Basic

Containers for Collection, Storage and Preparation of Blood
3DMed

IL-36RA, human
Bio Basic

IL-22, mouse
Bio Basic

Bone Morphogenetic Protein-4 ; human BMP-4
Bio Basic

INDICAID Antigen Rapid Test (Nasal Test Kit)

2xYT Broth
Bio Basic

ELISA Plate Reader

Nutrient Agar
Bio Basic

YT Agar
Bio Basic

human MIP-4/PARC (CCL18)
Bio Basic

2xYT Agar
Bio Basic

human MCP-4 (CCL13)
Bio Basic

Omicron Variant Detection Kit
3DMed

T4 DNA Ligase (40000U)
Bio Basic
![Ethidium bromide [EB], 0.625MG/ml Stock Solution Ethidium bromide [EB], 0.625MG/ml Stock Solution](https://cdn11.bigcommerce.com/s-i3vkl74ckd/images/stencil/590x590/products/1171/1489/bio-basic__43268.1641692136__81459.1641692351__11014.1641965546.png?c=1)
Ethidium bromide [EB], 0.625MG/ml Stock Solution
Bio Basic