GMD5501-GMP 50kU (200 μL, 250U/μL)

• Obtain consistent results.
  Rely on the robust reaction performance and the lot-to-lot consistency of this product.
• Stay ahead of regulatory requirements.
  High quality manufacturing, quality control and documentation according to GMP (Good Manufacturing Practice) regulations.

Use Taq DNA Polymerase, GMP Grade, 5 U/μl, for:

• Routine PCR and RT-PCR applications
• Amplification of DNA fragments up to 3 kb from various sources of DNA
• Labeling of DNA with modified nucleotides (e.g., DIG-dUTP, biotin-dUTP, fluorescein-dUTP)
• Combination with dUTP and Uracil-DNA Glycosylase for prevention of carryover contamination between PCR reactions
• Manufacture of amplification mixtures for applications with regulatory requirements (e.g.,in vitro diagnostics, quality control)

Taq DNA Polymerase is the recombinant full-length version of the thermostable enzyme from the eubacterium Thermus aquaticus BM, expressed in E. coli.
Enzyme acivities: Highly processive 5'-3' DNA polymerase; double-strand specific 5'-3' exonuclease; no 3'-5' exonuclease activity
pH optimum: Approximately 9.0 (+20°C)
Temperature optimum: Approximately +75°C
Half life at +95°C: Approximately 40 minutes
Substrates: Incorporates dNTP, dUPT, dITP, various labeled or modified nucleotides (200 μmol/L each is recommended of normal dNTP, increased concentrations of variants)
Divalent ion requirement: Mg²⁺ (1.5 mmol/L standard concentration)