SBI
ExoQuick® Exosome RNA Column Purification Kit
- SKU:
- EQ808A-1
- UPC:
- MPN:
- Availability:
- Usually Shipped in 5 Working Days
- size:
- 20 Preps
Description
Overview
How It Works
Simply add the phenol-free exosome lysis buffer to your isolated exosomes and then run through the RNA purification columns. In just three minutes, your highly pure exoRNAs will be ready for the next step, whether it’s processing for qPCR, microanalysis, NGS, or another application.
Supporting Data
Better qPCR profiling with the ExoQuick Exosome RNA Column Purification Kit
Figure 1. Serum RNA prepared by the SeraMir Kit delivers more reliable, reproducible qPCR profiles than when the RNA is isolated using conventional Trizol methods. Profiling of 380 Human microRNAs across the SeraMir 384 Profiler. The phenol-free exosome lysis step coupled to the small RNA binding columns isolates exoRNAs with much higher purity than Trizol/Phenol based methods. The SeraMir exoRNAs are compatible with downstream polyadenylation and reverse trancription reactions for amplification and accurate qPCR profiling.
Figure 2. Serum exoRNAs prepared using ExoQuick Exosome RNA Column Purification Kit deliver excellent performance in microarray studies. Samples from a pooled normal serum preparation and from a male caucasian (age 73) with adenocarcinoma of the colon were used in this study. Exosomes were precipitated from 250 µL of serum using the ExoQuick Exosome RNA Column Amplification Kit. The T7-amplified “sense” exoRNAs were then used for direct labeling analyses on LC Sciences miRBase ver.16 array chips (performed in triplicate). The exoRNAs were hybridized across 1,214 different microRNAs on the probe set. Of the 1,214 microRNAs analyzed, 79 microRNAs showed a signal intensity >32. Within this set of 79, there was a clear colon versus normal “signature set” of 40 microRNAs that could discriminate normal from colon cancer serum samples with a p-value < 0.01. The identities of the microRNAs found in this study have been masked while further investigation continues.
Related Products

Automatic RNA Extraction Kit by Magnetic Beads (12x96 purifications)
3DMed

EVeryRNA™ EV RNA Purification System with ExoQuick®-TC EV Isolation
SBI

ExoQuick® Exosome Isolation and RNA Purification Kit (for Tissue Culture Media)
SBI

ExoQuick® Exosome Isolation and RNA Purification Kit (for Serum & Plasma)
SBI
Customers Also Viewed

ExoQuick® Exosome Isolation and RNA Purification Kit (for Tissue Culture Media)
SBI

EVeryRNA™ EV RNA Purification System with ExoQuick®-TC EV Isolation
SBI

NA™ EVEVeryR RNA Purification System with ExoQuick® EV Isolation
SBI

ExoQuick® Exosome Isolation and RNA Purification Kit (for Serum & Plasma)
SBI

Automatic RNA Extraction Kit by Magnetic Beads (12x96 purifications)
3DMed

Rapid Animal Total RNA Extraction Kit
Bio Basic

Rapid Fungal RNA Extraction Kit
Bio Basic

Rapid Viral RNA Extraction Kit
Bio Basic

ExoQuick® ULTRA EV Isolation System
SBI

ExoQuick-TC® ULTRA for Tissue Culture Media
SBI

ExoQuick Plasma Prep with Thrombin
SBI

ExoQuick-TC PLUS
SBI

ExoQuick-TC
SBI

The Original ExoQuick
SBI

Zip 24 Surgical Skin Closure Device
Zip Medical

ExoQuick-LP
SBI

ExoQuick-CG
SBI

rProtein G Beads 4FF
Bio Basic

Vacutainer blood collection tube

Active Transfer Medium HANKS Buffer Based
Bio Basic

ELISA Plate Reader

ExoQuick PLUS
SBI

Xylenol orange
Bio Basic

Automated Nucleic Acid Extraction System
3DMed

GMD5501-GMP 50kU (200 μL, 250U/μL)

YT Agar
Bio Basic

HemogloBind™ Hemoglobin Depletion From Hemolyzed Serum/Plasma

HYLB Broth
Bio Basic

Xylene cyanol FF
Bio Basic

EZ-10 Spin Column Blood Genomic DNA Mini Preparations Kit
Bio Basic

CMV IgM Elisa
Ratio Diagnostics

SARS-CoV-2 Detection Kit by qPCR – ANDIS
3DMed

rProtein L Beads 4FF
Bio Basic

TBST buffer 10x Strength Solution
Bio Basic

Inactive Transfer Medium, Preservative Buffer
Bio Basic

Sodium phosphate, monobasic, monohydrate
Bio Basic

G 418 sulfate,50mg/ml solution Sterilized
Bio Basic

Eco-White-DNA Dye, ready to use
Bio Basic

EZ-10 Spin Column Yeast Genomic DNA Mini- Preparations Kit
Bio Basic

UnScience Antigen Test 25 tests
GENTAUR

GMD3501, 3502 , 3503 Nucleic Acid Extraction Kits

Containers for Collection, Storage and Preparation of Blood
3DMed

Rapid Detection Kit for Covid-19 by qPCR
3DMed

INDICAID Antigen Rapid Test (Nasal Test Kit)

100-1500bp DNA Marker, Ready-to-use
Bio Basic

25-500bp Low Range DNA Marker A, Ready-to-use
Bio Basic

YM Agar
Bio Basic

Peptone E GELATIN Peptone
Bio Basic

Streptavidin Beads 6FF
Bio Basic

PBST buffer 10x Strength Solution, Sterile
Bio Basic