ExoQuick® Exosome Isolation and RNA Purification Kit (for Tissue Culture Media)

Better qPCR profiling with ExoQuick Exosome Isolation and RNA Purification Kit (for Tissue Culture Media)

Figure 1. Serum RNA prepared by the ExoQuick Exosome Isolation and RNA Purification Kit (for Tissue Culture Media) delivers more reliable, reproducible qPCR profiles than when the RNA is isolated using conventional Trizol methods. Profiling of 380 Human microRNAs across a custom 384-well microRNA profiler array. The phenol-free exosome lysis step coupled to the small RNA binding columns isolates exoRNAs with much higher purity than Trizol/Phenol based methods. The ExoQuick exoRNAs are compatible with downstream polyadenylation and reverse transcription reactions for amplification and accurate qPCR profiling.

Figure 2. Serum exoRNAs prepared using ExoQuick Exosome Isolation and RNA Purification Kit (for Tissue Culture Media) deliver excellent performance in microarray studies. Samples from a pooled normal serum preparation and from a male caucasian (age 73) with adenocarcinoma of the colon were used in this study. Exosomes were precipitated from 250 µL of serum using the ExoQuick Exosome Isolation and RNA Purification Kit (for Tissue Culture Media). The T7-amplified “sense” exoRNAs were then used for direct labeling analyses on LC Sciences miRBase ver.16 array chips (performed in triplicate). The exoRNAs were hybridized across 1,214 different microRNAs on the probe set. Of the 1,214 microRNAs analyzed, 79 microRNAs showed a signal intensity >32. Within this set of 79, there was a clear colon versus normal “signature set” of 40 microRNAs that could discriminate normal from colon cancer serum samples with a p-value < 0.01. The identities of the microRNAs found in this study have been masked while further investigation continues.