Cygnus Technologies
KANEKA KanCapA™ 3G Protein A Mix-N-Go ELISA Kit | F950
- SKU:
- 214-F950
- UPC:
- MPN:
- Size:
- 1 Kit
- Product Type:
- ELISA Kit
- Storage:
- All kit reagents 2°C to 8°C
- Assay format:
- 96 Well Plate
- Time to result:
- ~1 hr. 45 min
- LOD:
- ~20 pg/mL
- LLOQ:
- ~0.16 ng/mL
- Recommended Diluent:
- I600
Description
KANEKA KanCapA™ 3G Protein A Mix-N-Go ELISA Kit | F950
KANEKA KanCapA™ 3G Protein A Mix-N-Go ELISA Kit is intended for use in quantitating the recombinant Protein A ligand using the KANEKA KanCapA™ 3G chromatographic resin. The kit is for Research and Manufacturing Use Only and is not intended for diagnostic use in humans or animals. The Mix-N-Go assay for KANEKA KanCapA™ 3G Protein A is a two-site immunoenzymetric assay. Samples containing KANEKA KanCapA™ 3G Protein A ligands are first diluted in the Mix–N-Go Sample Diluent provided with the kit. The Mix-N-Go Denaturing Buffer is then added and mixed to dissociate the Protein A from the product antibody. The samples are then reacted in microtiter strips coated with a polyclonal anti-Protein A capture antibody. A second anti-Protein A antibody labeled directly with Horse Radish Peroxidase (HRP) enzyme is simultaneously reacted forming a sandwich complex of solid phase antibody-Protein A:HRP labeled antibody. After a wash step to remove any unbound reactants, the strips are then reacted with Tetramethylbenzidine (TMB) substrate. The amount of hydrolyzed substrate is read on a microtiter plate reader and will be directly proportional to the concentration of Protein A present in the sample. Accurate quantitation is achieved by comparing the signal of unknown samples and controls to KANEKA KanCapA™ 3G Protein A Standards assayed at the same time.
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