GH7001 BstDNA Polymerase 1.0

The original polymerase for Loop-Mediated Isothermal Amplification (LAMP)

• Modified to retain 5´ → 3´ polymerase activity while lacking 5´ →3´ exonuclease activity
• Suitable for applications requiring thermophilic strand displacement
• Supplied with ThermoPol^® Reaction Buffer for high product yields in demanding conditions

Nucleic acid amplification techniques are widely used in infectious disease diagnosis, genetic traits, and other clinical practitioners in application-oriented fields. However, the traditional PCR procedure is time consuming and requires expensive special equipment that is rarely available in rural and underdeveloped areas. Recently, Notomi et al. developed a rapid detection of nucleic acid bases, loop-mediated isothermal amplification (LAMP), which with high sensitivity and specificity successfully achieved the amplification of the target DNA sequence at constant temperature in a water bath for one hour regardless the normal cycles of three temperatures [1, 2]. The LAMP technique has been widely used in the detection of viruses for infectious diseases, such as avian influenza [3], HFMD [4], dengue virus [5], human immunodeficiency virus [6] and virus of Ebola [7].

At present, a new simple colorimetric isothermal multiple self-adaptation (IMSA) initiated amplification has been developed to achieve rapid detection of EV71 in the early HFMD phase through visual color change by addition of hydroxyl naphthol blue dye [ 8]. Similar to the LAMP assay, the IMSA assay is an in vivo nucleic acid amplification technique that relies on Bst DNA polymerase, dNTP, magnesium betaine ions, and three primer pairs consisting of a parent primer pair (SteF and SteR), an outer primer pair (DsF and DsR) and an inner primer pair (FIT and RIT). Compared to the LAMP assay, the primer design for the IMSA assay has its own distinctive features that significantly lower the detection limits of the LAMP assay. To accurately perform quantitative analysis, the IMSA assay generated increased fluorescence in positive samples, allowing real-time monitoring detection.