Cygnus Technologies
SF9 HCP ELISA Kit 2G | F840
- SKU:
- 214-F840
- Size:
- 1 Kit
- Product Type:
- ELISA Kit
- Storage:
- All kit reagents 2°C to 8°C
- Target Expression System:
- SF9
- Species Group:
- Insect
- Species:
- S. frugiperda
- Assay format:
- 96 Well Plate
- Time to result:
- ~2 hrs. 50 min
- LOD:
- 0.3 ng/mL
- LLOQ:
- 0.5 ng/mL
- Recommended Diluent:
- I028
Description
SF9 HCP ELISA Kit 2G | F840
SF9 HCP ELISA Kit 2G is intended for use in determining the presence of host cell protein contamination in products manufactured by expression in SF9 insect host cells. The kit is for Research and Manufacturing Use Only and is not intended for diagnostic use in humans or animals. Users should validate this assay for use with their product samples. This kit is a 2nd Generation method replacing our earlier Catalog # F020, SF9 Insect Cell HCP ELISA kit. The SF9 Insect Cell Host Cell Protein assay is a two-site immunoenzymetric assay. Samples containing SF9 insect cell proteins are reacted in microtiter strips coated with an affinity purified capture antibody. A second HRP labeled anti-SF9 insect cell antibody is reacted simultaneously, forming a sandwich complex of solid phase antibody-SF9 HCP-enzyme labeled antibody. The microtiter strips are then washed to remove any unbound reactants. After the washes, the substrate tetramethylbenzidine is then reacted. The amount of hydrolyzed substrate is read on a microtiter plate reader and is directly proportional to the concentration of SF9 insect cell HCPs present.
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