Cygnus Technologies
JSR Protein A ELISA Kit | F740
- SKU:
- 214-F740
- UPC:
- MPN:
- Size:
- 1 Kit
- Product Type:
- ELISA Kit
- Storage:
- All kit reagents 2°C to 8°C
- Assay format:
- 96 Well Plate
- Time to result:
- ~1 hr. 45 min
- LOD:
- ~ 50 pg/mL ,or 25 pg/mL
- LLOQ:
- 100 pg/mL , or 50 pg/mL
- Recommended Diluent:
- I600
Description
JSR Protein A ELISA Kit | F740
JSR Protein A ELISA Kit is intended for use in quantitating the recombinant protein A ligands used in JSR Life Sciences’ AmsphereTM protein A chromatography resin series. It is for Research and Manufacturing Use Only and is not intended for diagnostic use in humans or animals. The Mix-N-Go assay for AmsphereTM Protein A is a twosite immunoenzymetric assay. Samples containing AmsphereTM Protein A ligands are first diluted in the Mix–N-Go Sample Diluent provided with the kit. The Mix-NGo Denaturing Buffer is then added and mixed to dissociate the Protein A from the product antibody. The samples are then reacted in microtiter strips coated with a polyclonal anti-Protein A capture antibody. A second anti-Protein A antibody labeled directly with Horse Radish Peroxidase (HRP) enzyme is simultaneously reacted forming a sandwich complex of solid phase antibody-Protein A: HRP labeled antibody. After a wash step to remove any unbound reactants, the strips are then reacted with Tetramethylbenzidine (TMB) substrate. The amount of hydrolyzed substrate is read on a microtiter plate reader and will be directly proportional to the concentration of Protein A present in the sample. Accurate quantitation is achieved by comparing the signal of unknowns to AmsphereTM Protein A Standards assayed at the same time.
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