See ExoQuick-LP performance data

With ExoQuick-LP, up to 90% depletion of lipoprotein particles from serum or plasma can be achieved. SBI’s exosome isolation products help you take full advantage of the power of exosomes.

ExoQuick-LP efficiently clears lipoproteins from serum

ExoQuick-LP treatment of serum results in lower amounts of lipoprotein markers (ApoA-1 for HDLs, and ApoE for LDLs) that co-isolate with exosomes than treatment of serum with ExoQuick alone, as shown by ELISA (Figure 1).

Figure 1. ExoQuick-LP efficiently clears lipoproteins from serum.

To test for exosome recovery after ExoQuick-LP pre-clearance, we used SBI’s Exosome ELISA kits (Cat.# EXOEL-CD81A-1, EXOEL-CD63A-1, and EXOEL-CD9A-1) to measure the approximate number of exosomal particles present in ExoQuick serum pellets with or without treatment with ExoQuick-LP (Figure 2). While there were some differences in observed particle numbers in untreated vs treated samples, overall yields were quite similar, suggesting minimal loss of material using ExoQuick-L

igure 2. ExoQuick-LP delivers robust exosome yields.

Serum exosomes pre-treated with ExoQuick-LP were also tested for size and concentration using NanoSight tracking analysis with a 1:100 dilution of isolated exosomes (Figure 3). We observed that there are minimal differences in particle sizes using ExoQuick-LP vs untreated controls, suggesting that exosomes are intact and structurally similar to exosomes isolated using standard ExoQuick isolation methods.

Figure 3. ExoQuick-LP delivers particles of the size expected for exosomes.