TruePrep Index Kit V2 for Illumina

1. 8 kinds of N5XX, 12 kinds of N7XX, can be used in combination to prepare 96 different paired-end Index libraries
2. Strict quality control and functional verification of each batch

TruePrep ® Index Kit V2 for Illuminadedicated companion kitTruePrep ® DNA Library Prep Kit V2 for IlluminaIt contains 8 kinds of N5XX and 12 kinds of N7XX, which can be used for the preparation of 96 different paired-end Index libraries. All reagents provided in the kit have undergone strict quality control and functional verification to ensure the stability and reproducibility of library construction to the greatest extent.

All components are stored at -30 ~ -15℃, and transported at ≤0℃

This is descriptive information
N411-01
VAHTS DNA Clean Beads

Q1: How many index combinations can TruePrep ^TM Index Kit V2 for Illumina ^® (Vazyme#TD202) provide?

A1: Provides up to 384 index combinations, including 8 N5XX and 12 N7XX.

Q2: Can I use VAHTS ^™ DNA adapters (Vazyme# N321/N322) instead of TruePrep ^™ adapters (Vazyme#TD202) when using TD501/502/503 for DNA library construction?

A2: No. The principle of adaptor ligation for transposase library building is different from that of mechanical interruption of library building.

Q3: Is there any difference between transposase library construction and on-machine sequencing after ordinary DNA library construction?

A3: There is a difference. The transposase adapter has a core activation sequence of 19bp, which is compatible with most Illumina sequencing platforms during the sequencing process. It is recommended that teachers consult the specific situation before sequencing.

TruePrep Index Kit V2 for Illumina

This is is specially designed for TruePrep DNA Library Prep Kit V2 for Illumina®
(Vazyme, #TD501, #TD502, #TD503).

It contains 8 kinds of N5XX and 12 kinds of N7XX, providing 96 kinds of different dual-indexed adapter combinations.
Special for TruePrep DNA Library Prep Kit V2 for Illumina®

(Vazyme, #TD501, #TD502, #TD503), providing 96 kinds of different dual-indexed adapter
combinations.

Version5.1
All the components can be stored at -20℃.
Storage
Vazyme Biotech Co., Ltd O

Components TD202 (96 rxn)

Contents of Kit
Application

16-Hour Incubation:

A50μl

reactionsystem

containing 5μ lof Oligosand 1μg of HindIII- λ DNA incubated at 37℃ for 16 hours resulted in no band
degraded detected by agarose gelelectrophoresis.

A 50 μl reaction systemcontaining 5μl of Oligosand 1μg of T3 DNA incubatedat 37℃ for 16 hours
resulted in no band degraded detected by agarose gel electrophoresis.

Endonuclease Activity:A50 μl reaction system containing 5μl of Oligos and 1μg of φX174RF I DNA incubated at 37℃ for 4 hours resulted in < 10%
conversion to RF II analyzed by agarose gel electrophoresis.
Quality Control
Index 2 (i5)
Index 1 (i7)
N5XX
N7XX
N501
N502
N503
N504
N505
N506
N507
N508
N701
N702
N703
N704
N705
N706
N707
N708
N709
N710
N711
N712
120 μl
120 μl
120 μl
120 μl
120 μl
120 μl
120 μl
120 μl
80 μl
80 μl
80 μl
80 μl
80 μl
80 μl
80 μl
80 μl
80 μl
80 μl
80 μl
80 μl
TAGATCGC
C T CT C TAT
TATC C TC T
AGAGTAGA
GTAAGGAG
ACTGCATA
AAGGAGTA
CTAAGCCT
TAAGGCGA
CGTACTAG
AGGCAGAA
TCCTGAGC
GGACTCCT
TAGGCATG
CTCTCTAC
CAGAGAGG
GCTACGCT
CGAGGCTG
AAGAGGCA
GTAGAGGA

Protocol

Strategy of Index Selection

Green fluorescent labeled dG/dT and red fluorescent labeled dC/dA were used in Illumina. To ensure successful sequencing, both green and red fluorescent signal must be detected in each sequencing cycle. Therefore, it is important to keep balance of the green and red fluorescence signals when choosing the indices.

The following are some recommended combination of the Indices:
The combinations of indices listed above are only some examples of acceptable selections. In practice, you can check the sequence of each index (refer to the first table) to make sure that there are two kinds of fluorescence signals at each base position, exemplified in the following table.

√: have both green and red fluorescence signals.
×: Missing green or red fluorescence signals.
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