Insulin ELISA Kit | F040

is intended for use in quantitating insulin from bovine, human or porcine sources. The kit is for Research and Manufacturing Use Only and is not approved for diagnostic use in humans or animals. For applications requiring lower limits of detection, Cygnus Technologies LLC manufactures an Ultra Sensitive Insulin ELISA kit, Catalog #F280. That kit has standards in the range of 25-1250pg/mL with a limit of quantitation of <25pg/mL. As such that kit can be used for testing insulin levels in serum from fasting individuals. This insulin assay is a two-site immunoenzymetric assay. Samples containing insulin are reacted simultaneously in microtiter strips coated with an affinity purified capture antibody. A second anti-Insulin antibody labeled alkaline phosphatase is reacted forming a sandwich complex of solid phase antibody- InsulinAlkaline phosphatase labeled antibody. After a wash step to remove any unbound reactants, the strips are then reacted with p-nitrophenyl phosphate (PNPP) substrate. The amount of hydrolyzed substrate is read on a microtiter plate reader and will be directly proportional to the concentration of Insulin present. Accurate quantitation is achieved by comparing the signal of unknowns to Insulin standards assayed at the same time.