Human Transferrin ELISA Kit | F035N

Human Transferrin ELISA Kit is intended for use in quantitating human transferrin. The kit is for Research and Manufacturing Use Only and is not intended for diagnostic use in humans or animals. The human transferrin assay is a two-site immunoenzymetric assay. Samples containing human transferrin are reacted in microtiter strips coated with an affinity purified capture antibody. A second HRP labeled anti-human transferrin antibody is reacted, forming a sandwich complex of solid phase antibody-transferrinHRP labeled antibody. After a wash step to remove any unbound reactants the strips are then reacted with TMB substrate, followed by the addition of a stop solution changing the color from blue to yellow. The amount of hydrolyzed substrate is read on a microtiter plate reader and will be directly proportional to the concentration of human transferrin present in the sample. Accurate quantitation is achieved by comparing the signal of unknowns human transferrin standards assayed at the same time.