Cygnus Technologies
BSA ELISA Kit | F030
- SKU:
- 214-F030
- Size:
- 1 Kit
- Product Type:
- ELISA Kit
- Storage:
- All kit reagents 2°C to 8°C
- Assay format:
- 96 Well Plate
- Time to result:
- ~1 hr. 50 min
- LOD:
- ~125 pg/mL
- LLOQ:
- ~250 pg/mL
- Recommended Diluent:
- F031A
Description
BSA ELISA Kit | F030
BSA ELISA Kit is intended for use in quantitating bovine serum albumin (BSA). The kit is for Research and Manufacturing Use Only and is not intended for diagnostic use in humans or animals. This BSA assay is a two-site immunoenzymetric assay. Samples containing BSA are reacted in microtiter strips coated with an affinity purified capture antibody. A second anti-BSA antibody labeled with the enzyme horseradish peroxidase (HRP) is reacted simultaneously forming a sandwich complex of solid phase antibodyBSA-HRP labeled antibody. After a wash step to remove any unbound reactants the strips are then reacted with tetramethylbenzidine (TMB) substrate. The amount of hydrolyzed substrate is read on a microtiter plate reader and will be directly proportional to the concentration of BSA present. Accurate quantitation is achieved by comparing the signal of unknowns to BSA standards assayed at the same time.
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