- Beyond the Design Guidelines, does Gen9 have any additional suggestions?
Yes, please ensure that your DNA sequences include any and all common flanking, promoter and/or endonuclease restriction site(s) required for manipulation.
- How do I place an order?
You can submit your sequences to Gen9 via email firstname.lastname@example.org as an attachment. Please refer to the Design Guidelines for specific details concerning the gene sequence design. The sequences should be transmitted using the Gen9 Sequence Submission Form (zipped Excel file). All polynucleotide construct sequences must be assigned a unique name, and should be transmitted to Gen9 as complete and final.
- What lengths of synthetic genes can you synthesize?
Gen9 currently has two product groups based on size, (1) GeneBits (500bp to 1000bp); (2) GeneBytes (1000bp to 3000bp). However, Gen9 has extensive experience synthesizing genes outside of this range. Please talk to your Gen9 sales representative for more information about orders outside of this range.
- How many genes can I order at one time?
Gen9 is a high-volume provider of synthesized genes and all gene-length constructs are provided to the customer in a 96-well plate(s). Each 96-well plate will contain 90 wells devoted to customer sequences, with the remaining wells used for manufacturing controls. For GeneBits, our minimum order size is one plate (please consult your sales representative for orders less than one plate). Gen9 has no problem fulfilling large orders.
- How does Gen9 make a synthetic gene construct?
Single-stranded oligonucleotides are designed, synthesized, and assembled using different protocols depending on the sequence characteristics. The assembled full-length construct is always verified by sequencing.
- How is quality assurance performed?
Gen9 utilizes a number of analytical methods, monitoring purity, yield and sequence fidelity, to assure that we are shipping a high quality product.
- How is my ordered sequence delivered?
The ordered sequence will be delivered as a blunt-ended non-phosphorylated linear dsDNA. The ordered sequence is contained between flanking 36bp regions. The flanking sequences are automatically appended during the synthesis process. If you would prefer to have the flanking region removed, please contact your sales representative.
- How are the synthetic genes shipped?
Gen9 will send your order in 96-well 500 µl deep-well plates shipped with freezer cool packs. The DNA material contained is shipped wet and eluted in 10 mM Tris-Cl, pH 8.5 buffer. The amount of shipped DNA material will vary from construct to construct. You will receive at least 50 ng of material for each shipped construct (often the amount of material will be >1 ug). Along with the synthetic gene, you will receive a packing list and a summary report which can be used to map the ordered customer sequences to a plate and well ID.
- Does Gen9 offer sequence design services, such as codon optimization?
Although Gen9 has helped customers with specific design issues and needs, we do not routinely offer design services, but please contact your sales representative if you have specific design needs.
- What is the price per base pair of a synthesized gene?
Because Gen9 employs an innovative, high throughput process to synthesize all of our genes and gene fragments we are able to offer the most competitive price currently possible.
- What is the error rate of the synthesized genes?
All of our GeneByte and GeneBit-Gold products have an error rate at the limit of detection of DNA sequencing. For customers interested in library screening applications, we offer our standard GeneBit products, which have an error rate of approximately one in every 2500 bases.
- When will Gen9 offer synthesized genes >3kb?
Gen9 has a number of research and development projects in process now that will lead to a numerous improvements as well as an expansion of our current product offering. We expect that by the end of 2013 we will offer synthesized genes up to 10kb.
- What is the turnaround time? Is this the same for large orders?
Gen9 strives for consistency in our product as well as our turnaround time. Currently we ship our synthesized genes in 20 business days after accepting the order.
- Can I get my genes cloned into a vector?
Both our GeneBits and GeneBytes are generally shipped as linear dsDNA. If you would like your synthesized genes cloned into a vector please contact your sales representative.
- Does Gen9 offer synthesized variant libraries?
Gen9 has a number of research and development projects in process now that will lead to numerous improvements as well as an expansion of our current product offering. We expect that by the end of 2013 we will offer degenerate libraries.